Technique / Molecular Biology / DNA-protein interactions / DNase I footprinting assay


DNase I Protection



DNase I Protection

A short protocol on DNase I protection from Molecular Biology of Nuclear Receptors, France.

You will have first to determine the concentration of DNaseI that cleaves
30-50% of your input DNA. A rule of thumb is to multiply this amount by 10 to get the adequate concentration of enzyme required to digest the DNA in presence of nuclear extract. See also the alternate protocol.

Last update 15-Dec-2005, Rating of 1 votes.

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By Raaj on 15-Dec-2005
Sir I am performing DNase I footprinting with 300bp fragment.This fragment is amplified by using 5' end labeled (T4 pol Kinase,p32Gama ATP)forward primer and unlabeled reverse primer,in this way i have 5' end labeled (at one end)300bp fragment for footprinting experiment.My DNA+protein complex is getting digested but lot of DNA getting structed in the wells
How i get ride of that?
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