Isabel’s Magical Cloning Protocol

Gene cloning protocol from Lahn lab at University of Chicago.

1. Insert amplification.
2. Vector/Insert digestion and Vector dephosphorylation.
3. Ligation.
4. Transformation.
5. Colony screening.
6. Mini-prep positive clones and sequencing.

Last update 12-May-2005, Rating of 0 votes.

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