cDNA Synthesis with Oligo dT Primers: First strand synthesis

A pdf protocol from Lahn lab at University of Chicago.

This may be done with total RNA or poly-A RNA prepped from
cell. Here one copy of cDNA will be made with a poly-T primer. (May isolate
total RNA and then prep mRNA by selection with oligoT beads to bind poly-A
tail and then make cDNA from this. However, poly-T primer should extend
only the mRNA from the total RNA prep).

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