Technique / Molecular Biology / PCR / 5' and 3' RACE
cDNA Synthesis with Oligo dT Primers: First strand synthesis
cDNA Synthesis with Oligo dT Primers: First strand synthesis
A pdf protocol from Lahn lab at University of Chicago.
This may be done with total RNA or poly-A RNA prepped from
cell. Here one copy of cDNA will be made with a poly-T primer. (May isolate
total RNA and then prep mRNA by selection with oligoT beads to bind poly-A
tail and then make cDNA from this. However, poly-T primer should extend
only the mRNA from the total RNA prep).
Last update 12-May-2005, Rating of 0 votes.
