Technique / Molecular Biology / Cloning library construction and screening


Typical Cloning Strategies and Tips



Typical Cloning Strategies and Tips

Some useful tips and strategies for DNA cloning. (From Mullin's lab at UCSF)

Last update 07-Dec-2007, Rating of 2 votes.

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By siddartha on 07-Aug-2008
i want to know the exact meaning of cloning from the basics... I want to know the cloning strategies...
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By Basha on 07-Dec-2007
i wanted to clone of 5.7 kb fragment in pGEMT easy vector.eventhough iam getting white colonies there was no insert only vector band was observed. my gene has NdeI at 5' end and hindIII at 3'end so i selected pET23a vector which has the same sites and ligated in 1:3 (vector:insert) but iam not getting waht may be the reason can u plz guide me to obtain clone of my gene of interest
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