Technique / Molecular Biology / Cloning library construction and screening
Cloning DNA for sequencing
Cloning DNA for sequencing
Protocol for cloning DNA for sequencing. (University of Southampton, School of Biological Sciences)
PURIFYING DNA FROM AN AGAR GEL
PHOSPHORYLATING PCR PRODUCTS
LIGATION
PREPARATION OF THE LB-AGAR AND SOC
TRANSFORMATION
PICKING POSITIVE COLONIES
SNAP FREEZING
ALKALINE LYSIS
TEST FOR INSERTION
Last update 12-May-2005, Rating of 0 votes.
Post your message in Cloning library construction and screening forum
Related resource
Manual Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Fo
Some vector sequence and map

Protocols for ET cloning

High throughput recombination cloning in E. coli

Genomic cloning troubleshooting

Subcloning oligos into expression vectors

CLONING PROBLEMS

cDNA library screening with antibody

Promotions