Technique / Molecular Biology / Cloning library construction and screening


CLONING PROBLEMS



CLONING PROBLEMS

I am making a contructor with pET vector; the question is:
I ligate the vector with the insert and all looks nice, when I transform
E.Coli and I make a mini-prep ( home mini-prep or kit mini-prep from
quiagen ) the plasmid tha appear is littlest than the normal ( molecular
mass 1Kb, when it should be of 6 Kb ). It don´t occurre all the time, at ... ...

Last update 16-Feb-2002, Rating of 0 votes.

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By poopdeloop on 16-Feb-2002
cloning is bad and i think that they should stop!

please give me more info. on problems with

cloning.
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By poopdeloop on 16-Feb-2002
cloning is bad and i think that they should stop!

please give me more info. on problems with

cloning.
Rating:

Reply
By poopdeloop on 16-Feb-2002
cloning is bad and i think that they should stop!

please give me more info. on problems with

cloning.
Rating:

Reply


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