Technique / Molecular Biology / RNAi siRNA gene silencing
Create siRNA Expression Cassettes by PCR
Create siRNA Expression Cassettes by PCR
Introduction of SECs from Ambion. SECs are PCR products that consist of promoter and terminator sequences flanking a hairpin siRNA template. Upon transfection into cells, the hairpin siRNA is expressed from the PCR product and induces gene specific silencing. (Ambion)
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