Technique / Molecular Biology / PCR / Standard PCR
Unknow Band from PCR product
Unknow Band from PCR product
I have run my PCR products on a 1% agarose gel with TAE buffer. However, I
found a strong band migrating towards the negative electrode after EBr stain
the gel. I still saw my product at the positive end that is apparently very
normal. ... ...
Related resource
PCR Optimization: Reaction Conditions and Components
High molecular weight PCR artefact

PCR trouble shooting, help, suggestions

Paraffin embedded tissue PCR

Standard PCR Protocol

PCR smearing trouble shooting

dNTP problem and magnesium Mg concentration in PCR reaction

PCR contamination: amplification in negative control

Promotions