Technique / Molecular Biology / RNAi siRNA gene silencing


siRNA design tools from Whitehead Institute



siRNA design tools from Whitehead Institute

This site helps you select siRNAs to knock down your gene of interest. After entering your sequence or accession/gi number, choosing pattern for your oligo nucleotides and various filter methods, you will be presented with a list of oligos matching your criteria. From this list, select those oligos you would like to consider further. All the selected oligos will be BLASTed against RefSeq, UniGene, or Ensembl database. In the BLAST result page, siRNAs can be further filtered to reduce the non-specific target effects. (WhiteHead Institute)

The site requires free registration.

Last update 29-Aug-2005, Rating of 3 votes.

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By siRNA on 27-Mar-2007
A free review paper on siRNA design:

Kurreck J. Free in PMC siRNA Efficiency: Structure or Sequence-That Is the Question.
J Biomed Biotechnol. 2006;2006(4):83757.
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By Vivek Vikram Singh on 06-Nov-2006
I really think this tool will help me in my project work
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By vgmalathi on 29-Aug-2005
excellent facility
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