siRNA validated and predicted sequence database

Nucleic Acids Res. 2005 January 1; 33(Database Issue): D131–D134.

siRNAdb: a database of siRNA sequences
Alistair M. Chalk,* Richard E. Warfinge, Patrick Georgii-Hemming, and Erik L. L. Sonnhammer

Center for Genomics and Bioinformatics, Karolinska Institutet, Berzelius väg 35, S-171 77 Stockholm, Sweden

Abstract

Short interfering RNAs (siRNAs) are a popular method for gene-knockdown, acting by degrading the target mRNA. Before performing experiments it is invaluable to locate and evaluate previous knockdown experiments for the gene of interest. The siRNA database provides a gene-centric view of siRNA experimental data, including siRNAs of known efficacy and siRNAs predicted to be of high efficacy by a combination of methods. Linked to these sequences is information such as siRNA thermodynamic properties and the potential for sequence-specific off-target effects. The database enables the user to evaluate an siRNA's potential for inhibition and non-specific effects. The database is available at http://siRNA.cgb.ki.se.

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