Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec
Rapid ethanol-based Coomassie Blue staining of SDS-polyacrylamide gels
Rapid ethanol-based Coomassie Blue staining of SDS-polyacrylamide gels
A staining protocol using ethanol instead of methanol from NWFSC/Molecular Biology Protocols. It also works well with methanol-based stain and destain, but significant amounts of methanol vapor are released into the laboratory---the ethanol is safer and just as effective. (Roger Rowlett of Colgate University)
Last update 07-Jun-2005, Rating of 0 votes.
Post your message in protein gel staining and protein digestion for mass spec forum
Related resource
Coomassie Blue: destaining
Precautions on gel preparation and staining

What if silver stain does not work?

Silver staining of SDS-PAGEs

Silver Staining (and De-Staining) of Polyacrylamide Gels

PAGE gel staining and troubleshooting

silver staining protocol from Breeden lab

Reduction/Alkylation and Proteolysis of Electrophoretically Separated Proteins

Promotions