Coomassie Stain Protocol

A nice protocol from Mass Spectrometry core facility at Ohio State University (in MS-word doc format).

The gel must be fixed prior to staining by a non-modifying, precipitation procedure such as the ethanol (or methanol)-acetic acid method used here. If the protein is not fixed in the gel as a separate step from the staining, the protein will be washed away and your results will be compromised. This is especially important for low level digests. (MS core, Ohio State University)

Last update 07-Jun-2005, Rating n/a of 0 votes.