Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec


PAGE gel staining and troubleshooting



PAGE gel staining and troubleshooting

This protocol and troubleshooting guide gives some troubleshooting on silver staining of polyacrylamide gel. (Frank lab, Oklahoma Medical Research Foundation)

1. Gray or brown precipitate appearing as smudges or swirling on gel surface. Bands may be faint or absent.
2. Dark uniform background, usually yellow.
3. Mottled background, usually brown or green, with poor sensitivity.
4. Slow or no development.

Last update 06-Jun-2007, Rating of 3 votes.

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By Rajiv Dua on 30-Dec-2008
Ya background truns yellow due to improper washing step after keeping the gel in fixative the is deposition of acidual residue to remove that keep the gel for longer time for wash..it will help u..try it...
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By Susan Thomas on 17-Mar-2008
I think it is because the pretreatment did not was out completely. I had the same problem before. I increased the washing step in between pretreatment and silverstaining
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By neethirajan on 06-Jun-2007
After silver stain the gel turn yellow, and no sharp bands
why this happens?
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