TRAP assay (Telomere Repeat Amplification Protocol)
|
TRAP assay (Telomere Repeat Amplification Protocol)
A pdf protcol on telomerase TRAP assay. (Lorel Colgin, Children's Medical Research Institute, Australia)
The TRAP assay is described in Kim et al. (1994), Science 266: 2011-2015, except that there is no incorporation of radioactive nucleotides nor end-labelling of primers • Principle: the assay measures enzymatic activity of telomerase if it is present. The M2 primer (also known as TS) acts as a substrate for telomerase-mediated addition of TTAGGG repeats. Addition of the repeats occurs at 25ˇăC, after which PCR is used to amplify the extended products. The M2 primer serves as forward primer while CX is the reverse primer for PCR. The products are then electrophoresed on a polyacrylamide gel, which is then stained with Sybergreen (Molecular Probes) to visualize the 6 bp ladder.
Telomere Repeat Amplification Protocol (TRAP)
From: TRAP and TRF assay protocols
Archived page
Last update 14-Jul-2005, Rating Good of 10 votes.
|
Write your comment
|
respeted sir,
i have been diong TRAP assay for the last 8-10 times..so far i was preparing the 10X REACTION BUFFER by myself..i have been used DEPC water to prepare that...still i havent got proper results...what might be the reason sir?
Rating: n/a
Reply
|
|
|
telomeres=bits of cell that get shorter every time cell divides. so im thinking shoelaces; each pair pf shoes, you cut laces in half to lace up a new pair, OK? after N new pairs, laces to short to tie any more --- > no more new shoes ... so the problem with cells just dividing forever would cause cancer? im guessing .. yes .. thanx Emma
Rating: Excellent!
Reply
|
|
|
sir,
i'm doing my graduation.As a part of my project i'm planning to perform TRAP assay.could u be kind enough to send a detailed protocol for TRAP assay?
thank u.
Rating: n/a
Reply
|
|
|
Dear Sir/Mam
With regard,I Meraj persuing PhD.For my research work I need detail protocal for TRAP essay either by manual or through kit ( with company specification).
I will be highly thankful for your kind help.
Thanks
Rating: Very Good
Reply
|
|
|
Please send me more info about the TRAP assays escpecially from Plasmodium falciparum
Rating: Very Good
Reply
|
|
|
Please send me more information about the trap assay. thank you very much!
Rating: n/a
Reply
|
|
|
i needed some more informations regarding trap assay. therefore requesting to kindly send me some TRAP assay related papers.Thankyou.
Rating: Excellent!
Reply
|
|
|
i am very interst know about the trap asay.pleaae sent me more valuable paper regarding this
Rating: Very Good
Reply
|
|
|
Please send me more info about the TRAP essays.
Thankyou!
Rating: Very Good
Reply
|
|
|
Science is beautiful in its simplest form and you are maintaining it .Yours is the best site I have seen in years
Rating: n/a
Reply
|
|
Related resource
Nonradioactive Telomere Length Assay
A Rapid Assay for Telomerase Activity on Telomeres
TRF Assay: Preparations for a Southern to determine telomere lengths
TelDB: Human Telomere Information database
Telomere measurement by quantitative PCR
Telomere length determination
Telomere Length Measurement by Fluorescence In Situ hybridization and flow cytometry
TRAP assay setup (Telomere Repeat Amplification Protocol)
|
