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TRAP assay (Telomere Repeat Amplification Protocol)



TRAP assay (Telomere Repeat Amplification Protocol)

A pdf protcol on telomerase TRAP assay. (Lorel Colgin, Children's Medical Research Institute, Australia)

The TRAP assay is described in Kim et al. (1994), Science 266: 2011-2015, except that there is no incorporation of radioactive nucleotides nor end-labelling of primers • Principle: the assay measures enzymatic activity of telomerase if it is present. The M2 primer (also known as TS) acts as a substrate for telomerase-mediated addition of TTAGGG repeats. Addition of the repeats occurs at 25ˇăC, after which PCR is used to amplify the extended products. The M2 primer serves as forward primer while CX is the reverse primer for PCR. The products are then electrophoresed on a polyacrylamide gel, which is then stained with Sybergreen (Molecular Probes) to visualize the 6 bp ladder.

Telomere Repeat Amplification Protocol (TRAP)
From: TRAP and TRF assay protocols

Last update 14-Jul-2005, Rating of 11 votes.

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By Dr. squall on 24-Feb-2009
i am very intersted to know about the trap assay. could you please sent me more valuable paper regarding this.
best regards

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By KIRAN K G on 05-Aug-2007
respeted sir,
i have been diong TRAP assay for the last 8-10 times..so far i was preparing the 10X REACTION BUFFER by myself..i have been used DEPC water to prepare that...still i havent got proper results...what might be the reason sir?
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By Cathy on 08-Jan-2007
telomeres=bits of cell that get shorter every time cell divides. so im thinking shoelaces; each pair pf shoes, you cut laces in half to lace up a new pair, OK? after N new pairs, laces to short to tie any more --- > no more new shoes ... so the problem with cells just dividing forever would cause cancer? im guessing .. yes .. thanx Emma
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By Aparna Ekbal on 04-Dec-2006
sir,
i'm doing my graduation.As a part of my project i'm planning to perform TRAP assay.could u be kind enough to send a detailed protocol for TRAP assay?
thank u.
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By Meraj on 01-Dec-2006
Dear Sir/Mam
With regard,I Meraj persuing PhD.For my research work I need detail protocal for TRAP essay either by manual or through kit ( with company specification).
I will be highly thankful for your kind help.
Thanks

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By Syamsudin on 13-Nov-2006
Please send me more info about the TRAP assays escpecially from Plasmodium falciparum
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By jinlong on 10-Oct-2006
Please send me more information about the trap assay. thank you very much!
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By Vijendra Kumar Mishra on 09-Oct-2006
i needed some more informations regarding trap assay. therefore requesting to kindly send me some TRAP assay related papers.Thankyou.
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By tom sinoy.e.s on 21-Sep-2006
i am very interst know about the trap asay.pleaae sent me more valuable paper regarding this
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By PIYUSH KUMAR JAIN on 13-Aug-2006
Please send me more info about the TRAP essays.
Thankyou!
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By DR KGAswini Kumar on 14-Jul-2005
Science is beautiful in its simplest form and you are maintaining it .Yours is the best site I have seen in years
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