ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS

Estimations of the numbers of nuclei in microtome sections are frequently made in some branches of histology, particularly in the study of endocrine glands, hemopoietic organs and the central nervous system. The curious neglect of this and indeed of all quantitative methods in most other fields of histology will no doubt soon be a thing of the past. It is therefore important to consider how best to get reliable conclusions from such nuclear counts. The present note deals with a commonly neglected difficulty which necessarily follows from the use of microtome sections. (M. ABERCROMBIE, Department of Zoology, The University of Birmingham, England)

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