Technique / Proteomics and protein biochemistry / Protein electrophoresis


protein electrophoresis problem



protein electrophoresis problem

I have a simple question, but it's hard for me.
I run SDS-PAGE using the protein samples prepared by myself, and stain the
gel with commassie blue after ecletrophoresis. The troublesome thing is that
there is always smear on the gel according to the place of each lane. I
don't know ... ...

Last update 11-Jul-2001, Rating of 4 votes.

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By waheed on 09-Nov-2006
why bands mix after running the electrrophoresis gel, as i laod them seprate
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By Sean on 05-Sep-2006
Did you check your running buffer? if it is right one for your gel type. Only use running buffer your gel instruction recommended.

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By Mel on 03-Sep-2006
I have seen this (a smear) when I load too much protein in a well. How much are you loading. Try loading less.
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By chandrasekaran.M, on 05-Mar-2006
i am running my protein samples last two weeks, upto now i didn't get any band in my gel, what is this reason

please give me some idiea for that i doing my research work regarding pathogenesis related proteins in tomato, after from that i protein is already purified my ammonium sulfate.

pls give me some clue
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By Renu Rawat on 11-Jul-2001
Are you adding bromophenol blue marker dye to each well, if yes it may be due that.Add marker dye only to first and last well


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