Conjugation of monoclonal antibodies

Protocol from M. Roederer. In this series of web pages, protocols, notes, and various illustrations are given to aid in the conjugation of proteins--principally monoclonal antibodies--to fluorescent dyes. These conjugation procedures are commonly performed in our laboratory--we have conjugated several hundred different monoclonals using almost all of the various dyes listed. The procedures are relatively straightforward and require only minimal familiarity with standard laboratory techniques (gel filtration and spectrophotometry are the most difficult!).

M. Roederer, Conjugation of monoclonal antibodies (August, 2004). http://www.drmr.com/abcon/

Last update 21-Jun-2005, Rating of 0 votes.

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