Technique / Molecular Biology / Enzymatic treatment of DNA/RNA
Labelling of oligos: an interesting phenomenon
Labelling of oligos: an interesting phenomenon
I had to label about 40-50 primers, and while doing this I have spotted a
strange phenomenon: the labelling efficiency could drop to less then 10%
depending on the first nucleotide of the primer, independently of the
sequence and condition. ... ...
Related resource
Double restriction digestion of DNA
Rsa digestion.

Non-radioactive labelling of DNA

High temp. restriction enzyme digests

RANDOM primer labelling efficiency

REBASER: The Restriction Enzyme Database

Integrated Enzyme Database (IntEnz)

NEBCutter 2.0 restriction map and ORF drawing

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