Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification

Preparation of Glycerol Stocks and Thawing of E.Coli Stocks

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By Peter Kim on 30-Dec-2005 This protocol does not make sense- there is an error either in the description or the protocol. It starts off by saying that the plasmids are unstable at glycerol conc >10% so the cells will be stored in 8%. Good so far. The protocol then calls for mixing 100ul of cells with 900ul of an 80% glycerol mixture. That would give a final concentration of 72%. I wonder if that should read 100ul of 80% glycerol with 900ul of cells. That would give a 8% final conc. Rating: Fair Reply