T4 DNA ligase frequently asked questions (FAQ)

This is a FAQ on T4 DNA ligase from New England Biolabs.

Q1: What are some potential problems with the ligation reaction using T4 DNA Ligase that can lead to transformation failure?
Q2: What are some other problems that should be considered when trouble shooting a transformation problem?
Q3: What problems can be encountered in the restriction digest that can cause ligation using T4 DNA Ligase or subsequent transformation to fail?
Q4: What controls should be run to test the cells and DNA when using T4 DNA Ligase?
Q5: When should T4 DNA Ligase be the enzyme of choice?
Q6: Can the T4 DNA Ligase be used with the Quick Ligase buffer?
Q7: How much DNA should be used in a ligation using T4 DNA Ligase?
Q8: Can T4 DNA Ligase be used in other NEBuffers?
Q9: Can T4 DNA Ligase be heat inactivated?
Q10: How do Weiss units convert into NEB cohesive end units?

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Last update 28-Jul-2005, Rating n/a of 0 votes.