Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification

Purification Tricks for Recombinant Proteins

Purification Tricks for Recombinant Proteins

An introduction of recombinant protein purification and application tricks by Bill Campbell at Michigan Tech.

Recombinant proteins are made from cloned DNA sequences which usually encode an enzyme or protein with known function. Special vectors (a vector is simply a tool for manipulating DNA and can be viewed as a versatile "carrying vehicle") are used for production of the proteins from specific DNA sequences cloned into them. Since purification of a protein can be a complex and time-consuming process, it is often possible to add a DNA sequence to the cloned protein's code which will append a small peptide or even a small protein for facilitating the purification of the recombinant protein after it is expressed.

Last update 28-Jan-2009, Rating of 1 votes.