Technique / Proteomics and protein biochemistry / Protein electrophoresis
SUMOplot: prediction of attachment of SUMO protein (11kDa) at multiple positions
SUMOplot: prediction of attachment of SUMO protein (11kDa) at multiple positions
SUMOplot™ can help you to explain larger MWs than expected on SDS gels due to attachment of SUMO protein (11kDa) at multiple positions of your protein. (Abgent Inc.)
SUMO-1 (small ubiquitin-related modifier; also known as PIC1, UBL1, Sentrin, GMP1, and Smt3) is a member of the ubiquitin and ubiquitin-like superfamily. Most SUMO-modified proteins contain the tetrapeptide motif B-K-x-D/E where B is a hydrophobic residue, K is the lysine conjugated to SUMO, x is any amino acid (aa), D or E is an acidic residue. Substrate specificity appears to be derived directly from Ubc9 and the respective substrate motif. SUMOplot™ is predicting the probability for the SUMO consensus sequence (SUMO-CS) to be engaged in SUMO attachment. The SUMOplot™ score system is based on two criteria: 1) direct amino acid match to the SUMO-CS observed and shown to bind Ubc9, and 2) substitution of the consensus amino acid residues with amino acid residues exhibiting similar hydrophobicity.
Last update 08-Sep-2005, Rating of 0 votes.
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