DNA cloning problem

i have succeeded in DNA cloning but the DNA fragment has errors during sequencing, my DNA fragment source is 1- PCR purified DNA 2- fragment subcloned from already prepared another plasmid
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We have been trying to clone a human gene in to TA vector. We've got
blue/white selection, have screened the white ones directly by PCR, and
then tried to grow up those with inserts - this is where the problem
starts. On trying to grow up for mini-preps, nothing happens. There is
absolutley no growth.
We have tried different media and both Kan and Amp selections (as well
as no antibiotics at all), but we just ... ...




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