Technique / Molecular Biology / RNAi siRNA gene silencing
RNAi Protocol Optimization Guide
RNAi Protocol Optimization Guide
A guidline for RNAi experiments design and protocol optimization. It is brought to you by BD bioscience with RNAi related products.
Content:
Getting Started
* I don’t know where to start
* I know which gene I’d like to “knockdown”. Now what?
Screening Your Knockdown Sequence
* I’d like to validate my shRNA sequence
* I need to screen a large number of potential knockdown sequences
* I’d like to use prevalidated knockdown sequences
* I’m not seeing good knockdown with the oligos or vector constructs that I’m using
Optimizing Gene Delivery
* I’ve achieved good knockdown with synthetic oligos. How do I get stable expression of my siRNA?
* I’m having difficulty transfecting my synthetic oligos or RNAi vector constructs
* I’m working with hard-to-transfect cell lines
Inducible Gene Silencing
* I’d like to use inducible gene silencing
Last update 25-Oct-2005, Rating of 0 votes.
RNA Interference of Gene Expression (RNAi) in Cultured Drosophila Cells
siRNA design tools from Whitehead Institute
siRNA sequence list (human, rat, mouse)
RNA Silencing Methods and Protocols
RNAi technique animation tutorial from Nature
siRNA transfection
Knock-down of endogenous proteins by RNA interference in HeLa cells
