Optimizing chemical transfection of chemically synthesized siRNA

One of the biggest hurdles to obtain reproducible gene silencing results using chemically synthesized siRNAs or DNA constructs that express siRNAs is developing an optimized transfection protocol. The condition for achieving the highest transfection efficiency involves several parameters including cell culture conditions, the composition of the siRNA/ liposomal complex and the duration of the transfection. (MoleculA, now a subsidiary of CalbaTech)

Last update 13-Dec-2005, Rating of 0 votes.

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