Electron microscopy protocols

Electron microscopy related protocols by Dr.Christen Harris, Medical College of Georgia.

Content:

Part A: Tissue Preparation

LTP Physiology Protocol
Vibroslicer VT 1000S
Slice Dissection and Vibratoming in Agarose
Stoelting Tissue Chopper
Microwave Processing Protocol
Schedules:
a. Standard Epon
b. Epon/Spurrs
c. Standard Epon with enhanced ribosomal
staining
Routine Embedding of Tissue
Re-embedding Tissue
Tissue Embedding for Photography
Tissue Preparation Safety Protocols
a. Protocol for Neutralizing the Aldehydes in our
Fixative Waste
b. Protocol for Disposing of Osmium Tetroxide
Contaminated Plastic Containers (solid osmium
tetroxide contaminated waste)
Part B: Microtomy

Glass Knife Making
Coating Grids
Denton Vacuum Carbon Evaporator
Photography of Epon Blocks using the Nikon Light Microscope
Hand Trimming Epon Blocks
Thick Sectioning
Thin Sectioning
Monitoring for Radioactivity
Staining Grids
Uranyl Acetate Recipe
Lead Citrate Recipe
Serial Sectioning
Ridge Pyramid Trimming for Serial Sectioning
Loading Serial Sections into Gimbols or Hex Rings
Part C: Electron Microscopy

Loading/Unloading Hex Rings into Gatan Rotational Holder
Loading/Unloading Gatan Rotational Holder into JEOL 2010
Loading/Unloading Gimbols into Rotational Holder
Loading/Unloading Rotational Holder into JEOL 1200 EX
Alignment Protocol for JEOL 1230
Photographing across the Depth of a Thin Section
Photographing Serial Sections
Unloading/Loading Film into the Electron Microscope
Developing Electron Microscope Film
Routine Scanning of EM Negatives Using Microtek Artix Scan 1800f Scanner
Scanning EM Negatives Using Polaroid SprintScan 45

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Last update 19-Jan-2006, Rating n/a of 0 votes.