Technique / Molecular Biology / Recombinant virus and gene therapy
Adenovirus Production Protocol
Adenovirus Production Protocol
A doc file of recombinant adenovirus production protocol. (Eric Kremer, Vect Euro Train)
Content:
I) Generation of the recombinantvector genome in a plasmid
II) Transfection of Recombinant Genome in transcomplementing cells
III) Preparation of an E1-deleted vector stock
IV) Purification of vector stock by banding on CsCl
V) Titration physical particles (by OD260) & PFUs (plaque forming units)
VIa) Titration of Infectious units
VIb) Infectious units assay by FACS
Last update 08-Feb-2006, Rating of 0 votes.
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