Technique / Genetics / Genotyping / Random Amplified Polymorphic DNA (RAPD) Technique


Random Amplified Polymorphic DNA (RAPD) Protocol



Random Amplified Polymorphic DNA (RAPD) Protocol

The Random Amplified Polymorphic DNA (RAPD) method is based on the Polymerase Chain Reaction (PCR) using short (usually 10 nucleotide) primers of arbitrary sequences. Polymorphism of amplified fragments are caused by: (1) base substitutions or deletions in the priming sites, (2) Insertions that render priming sites too distant to support amplification, or (3) insertions or deletions that change the size of the amplified fragment. (Bioinformatics Centre and Library, Central Plantation Crops Research Institute Kasaragod )

Last update 30-Apr-2008, Rating Good of 51 votes.

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