Random Amplified Polymorphic DNA (RAPD) Protocol
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Random Amplified Polymorphic DNA (RAPD) Protocol (archive)
The Random Amplified Polymorphic DNA (RAPD) method is based on the Polymerase Chain Reaction (PCR) using short (usually 10 nucleotide) primers of arbitrary sequences. Polymorphism of amplified fragments are caused by: (1) base substitutions or deletions in the priming sites, (2) Insertions that render priming sites too distant to support amplification, or (3) insertions or deletions that change the size of the amplified fragment. (Bioinformatics Centre and Library, Central Plantation Crops Research Institute Kasaragod )
Last update 28-Mar-2008, Rating Good of 46 votes.
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dear sir,
i am a masters of science in biology student here in the philippines. and i am currently writng a paper about Jatropha curcas. and i would just like to know of what is the first thing to do for extracting its DNA?.. its protocol.. RAPD is applicable right?.. but when it should be used then?
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Dear sir, i am doing M.sc biotechnology, I need some literature review of RAPD analysis in medicinal plants and i need protocol for RAPD.
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Dear Sir,
Thank a lot to u as u r providing assistance to research scholar. Iwant RAPD procedure along with materials and methods.I also want review of literature on RAPD for fresh water fishes.
Thanking you,
Rating: Excellent!
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Dear friend
i want to know about RAPD and use of your experiance to work with this method please help me
tanks
Rating: Excellent!
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Dear sir,
I would like to have RAPD protocol for running maize landraces being screened for drought tolerance.
Rating: Excellent!
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sir i am working on genetic diversity analysis of sorghum so i required standerdised protocol for DNA isolation and pcr amplification of sorghum bicolor moench. so i request for same
hoping for anticipation and favourable action thank you
Rating: Excellent!
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Dear sir,
I am a research scholar working on abiotic stess tolerance in pseudomonas. I will be greatful to you if you send me the materials and method for RAPD and review of literature on RAPD for abiotic stess tolerance genes in pseudomonas.
Thanking you,
Afroz
Rating: Excellent!
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sir
reques for your suggesion
sir i have plant having some antimalarial properties .now i want to go for some genetic lavel analysis of that plant .
with regardes
Rating: Excellent!
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Respected sir,
I am Vinoth. I am a research scholar. I am very much interested to isolate a character gene from Amaranthus plant. and what softwares to be used. can you guide me regarding the request. Can you send me d RAPD protocols.
Rating: Excellent!
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dear sir,ur effort are excellent , it is going to help no. of student who want to do research sicerely.I want complete protocol for RAPD in ashwagandha along with tje primers. Thank u.
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SIR,
I M A RESEARCH SCHOLAR IN B.Z.U.MULTAN.MY RESEARCH IS ON RICE GENETIC DIVERSITY ANALYSIS BY RAPD PCR.I APPRECIATE UR WORK AND UR SUPPORT TO STUDENTS KINDLY SEND ME PROTOCOLS OF RAPD PCR.I WILL BE GRATEFUL.
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I want to know about RAPD in details and also want protocol for sorghum DNA diversity
Rating: Very Good
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I want to know everything in details about RAPD. I need protocols to analise some fungi. Do you know how can I choose the primers?and the PCR cicle? Please, send me some information and IŽll be grateful to you. Thank you.
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Dear sir,
I want to know everything in details about RAPD and want RAPD protocol. i Will start RAPD Analysis in Jatropha having 33 genotypes. So if you send me this I will be grateful to you.
Rating: Excellent!
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Respected Sir,
First of all I would like to appreciate ur benevolent effort of helping students. Sir I searched a lot of literature but I couldnt find the exact primers for linseed RAPD. If you help me for this I shall be obliged.
Rating: Excellent!
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Dear sir
I want RAPD(Random amplified polymorphism DnA) protocol includ materials and methos.
thank you
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Respected sir
i wantRAPD procedure, along with method ,material and the primers specifically used for a cultivated mushroom Agaricus bisporus commonly known as button mushroom.i shall be very thankful to you.
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Does RAPD technique depend on only one primer or two primers as usual as normal PCR?
Thanks
Rating: Excellent!
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i need RAPD protocols as i have to osolate DNA from beetles and have to do their RAPD profiling
Rating: Excellent!
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Dear Sir,
I want to know the RAPD protocol for fingerprinting analysis of some tropical maize inbreds. Please suggest some primers which can generate good number of bands.
Thanks
Rating: Very Good
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Dear Sir,
I want to know the RAPD protocol for fingerprinting analysis of some tropical maize inbreds. Please suggest some primers which can generate good number of bands.
Thanks
Rating: Very Good
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Dear sir
I want RAPD(Random amplified polymorphism DnA) review of E.coli bacteria and protocol includ materials and methos.
thank you
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RAPD is based on the arbitarily designed (not based on the genome of the organism that we are doing RAPD) mixture of short (10-11bases) random primers that can virtually aneal to any location of the Genomic DNA. Its not only one set of primer that is used in the normal PCR.
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your suggetions are very useful to us ,
thanking u sir.
please send rapd primers for jatropha curcas
Rating: Excellent!
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Dear sir,
Does RAPD technique depend on only one primer or two primers as usual as normal PCR?
Thanks
Rating: Excellent!
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Dear sir I want RAPD procedure for groundnut late leaf spot...and review of literature.if you send am very grateful to you sir.
thank you
Rating: Fair
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Dear sir
i want RAPD (Randomly amplified polymorphism DNA) protocol, methods and also want to know how to calculate the pMol from micro gram concentration of RAPD primers.
Thank you
Rating: Excellent!
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Dear sir
I Want protocol of RAPD Plant genetic diversity analysis in sorghum.
Thanking You
Rating: Excellent!
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dear sir, i need RAPID protocol for jatropha along with details of its materials and methods required by it such as primers used, extraction buffer used, different conditions required during experiments
Rating: Excellent!
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Dear sir
I want RAPD protocol for Jatropha curcas which is an Biodiesel plant.
Rating: Excellent!
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