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A cytokine ELISA method animation



A cytokine ELISA method animation

A online animation on cytokine ELISA assay by C.T.L (BD Cellular Technologies Ltd).

Basic steps of cytokine ELISA assay:

1. Coat 96 well plate with cytokine capture antibody.
2. Add sample to capture cytokines and non-specific bound are washed off.
3. Add biotinylated antibody followed by HRP avidin to bound the cytokine-antibody complex.
4. Add substrate followed by colorimetric detection by spectrometry.

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Last update 23-May-2007, Rating Very Good of 1 votes.


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