Protocol for Fluoro-Jade Histofluorescent Labeling of Degenerating Neurons

This is a detailed protocol on application of fluoro-jade staining on degenerate neurons. (by Dr.Tom Hallam, University of California, Davis)

Fluoro-Jade can be used on almost any tissue section. From our experience FJ will stain any tissue section type or thickness: i.e. frozen sections, vibratomed sections, cryostat sections, or paraffin embedded sections from 3-50 mm. The staining protocol takes approximately 90 minutes and little prior histological experience is necessary. When FJ-stained section are viewed under a fluorescence microscope with a FITC filter, degenerating neurons fluoresce brightly in contrast to neighboring neurons. The staining protocol we use in our lab is almost exactly like that first described in Schmued et al (1997).

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Last update 17-Jun-2006, Rating n/a of 0 votes.