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Technique / Proteomics and protein biochemistry / Western blot assay


Western blot problem



Western blot problem (archive)

I have been having a lot of problems with efficiency of transfer from
gel (10% acrylamide/bis) to PVDF membrane (Immobilon P). When I stain
the gel after transfer, there is a lot of protein left on there. I know
that I am not exceeding the binding capacity of the membrane (I did a
titration of ug protein loaded and discovered that the ratio of protein
transferred to those that stay behind is the same).
The protein of interest is high moleculat weight (around 160
kDa). I have tried : ... ...

Last update 22-Jun-2001, Rating Fair of 8 votes.


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