Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification
protein precipitation
protein precipitation
I am looking for a protein precipitation method for very low
concentrated samples (50ug/ml) I have tried chloroform methanol but that
doesn't work. My samples are in phosphate buffer + n-octylglucoside. ... ...
Last update 22-Oct-2002, Rating of 11 votes.
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By Shanura Fernando
on 04-Nov-2008
this sight is verry usefull I think if you can view the results for those above questions it is more better |
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By Niru
on 05-Mar-2008
Go upto 500mM, 750mM and as high as 1M imidazole. it has worked for me with an insoluble HepC protein. |
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By Niru
on 05-Mar-2008
Did you leave it in 250mM imidazole overnight in the freezer...do not do that..dialyse after quickly running fractions on a gel...leaving protein in imidazole will cause protein to precipitate easily..N |
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By Syboo
on 04-Oct-2007
I want to precipitate my proteins that are already solved in Laemmli buffer. Can I use the common methods or is there a special one? |
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By Samy
on 15-Jul-2007
devi purificare degli enzimi da una soluzione salina attraverso la dialisi,ho bisogno di equazioni riguardanti la dialisi,qualcuno puņ aiutarmi? |
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By pintaka kusumaningtyas
on 19-Nov-2006
Dear bio net users |
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By Vijaypal Yadav
on 11-Apr-2006
My protein gets precipitate during dialysis within 2 hrs of dialysis. My protein is in phosphate buffer with 300mM NaCl and 250mM Imidazole, I am dialysing it against Trs-Cl buffer with NaCl 100mM. |
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By ratna
on 05-Feb-2006
hi all! |
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By ratna
on 13-Jan-2006
hi , |
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By conmar
on 11-Oct-2005
hello there. |
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By rubina
on 07-Oct-2005
Hi |
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By Vladimir Matveev
on 03-Mar-2004
Hand-to-Hand Model for Tropomyosin Fast Diffusion. |
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By hussain
on 20-Jun-2003
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By catia carias
on 07-Dec-2002
You can try to precipitate your peotein with a salt like ammonium sulphate our with a nonionic polymer, such as PEG. If you chose to work with PEG, you must decide with wiche PEG MW you should work, because it changes with the solutions. You can also try to add somo organic solvents... |
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By Kosalai K
on 22-Oct-2002
Can you try precipitating your samples with acidified actone (chilled). You can add 10 percent glacial acetic acid to the acetone & try precipitating with this solution such that the final concentration of acetone should be 80 percent in the sample. If you need to remove acetic acid, u can wash the pellet with methanol (60 percent) twice & dry the plle before use. hope it helps you..; |
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