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Technique / Proteomics and protein biochemistry / Protein assay


The Bradford Assay



The Bradford Assay

The bradford assay for protein concentration measurement (by Brad Olson, University of Nebraska-Lincoln)
Bradford assay uses the absorbance shift of Coomassie brilliant blue G-250 dye (595nm to 470nm) when bound to protein residues.
Reference: Bradford, M. M. (1976) Anal. Biochem. 72, 248

Bradford assay protocol for protein quantification (Stocknger lab, Ohio state university)

Wiki introduction of Bradford assay (Texas A&M University)

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Last update 22-Mar-2007, Rating n/a of 0 votes.


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