The BCA assay for protein quantification

Protocols and introduction on BCA assay for protein quantification:

BCA assay introduction and guide (John Markwell, University of Nebraska-Linkoln)
The BCA protein assay measures the formation of Cu+1 from Cu+2 by the Biuret complex in alkaline solutions of protein.

References:
P.K. Smith et al. (1985) Anal. Biochem. 150: 76.

K. J. Wiechelman et al. (1988) Anal. Biochem. 175: 231

BCA assay protocol (Oregon Health and Science University)

Pierce BCA Protein Assay Protocol (Pierce)

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Last update 09-Jun-2008, Rating Very Good of 1 votes.