Plasmid subcloning and ligation tips

Frequently asked questions on plasmid subcloning and DNA ligation from Promega. (Promega)

Subcloning and ligation tips on:

1. What parameters may need to be optimized for successful ligation reactions?
2. What controls are recommended to ensure successful subcloning?
3. What controls can be performed to test the competent cells?
4. What controls can be performed to test the ligation reaction?
5. What controls can be performed to test phosphatase treatment?
6. Are there any special considerations when performing T-cloning?
7. What is blue/white screening and how is it performed?
8. What factors govern the proper selection of host bacterial cell strain?
9. What other methods are available to screen recombinant colonies in addition to blue/white screening?

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Last update 17-May-2007, Rating n/a of 0 votes.