PCR cloning

PCR cloning protocols and methods review:

Considerations for PCR cloning (Excellent guideline for PCR cloning, Invitrogen)
Including tech hints for aspects of PCR cloning: insert and its size, vector-to-insert ratio, freshness of pcr products, compatibility of DNA ends of vector and insert, addition of 3'-A overhangs after PCR etc.

Chen's rough guide on PCR cloning

Simple One-Tube PCR Cloning Method (Chun-Ming Liu)

Cloning of PCR fragments (Michael P. Weiner)

Brief protocol for blunt end cloning of PCR products (HanCock Lab)

PCR cloning technique literatures:

1: Saito T, Dohmae N, Tsujimoto M, Takio K.
PCR cloning and heterologous expression of cDNA encoding a peptidyl-Lys
metalloendopeptidase precursor of Grifola frondosa.
J Gen Appl Microbiol. 2002 Oct;48(5):287-92.

2: Malo MS, Husain Z.
Positive selection vectors for high-fidelity PCR cloning.
Biotechniques. 2003 Jun;34(6):1250-8.

3: Kitabayashi M, Nishiya Y, Esaka M.
A simple and eficient method for high fidelity PCR cloning using
antibody-neutralizing technology.
Biosci Biotechnol Biochem. 2003 Sep;67(9):2034-7.

4: Anand RD, Sertil O, Lowry CV.
Restriction digestion monitors facilitate plasmid construction and PCR cloning.
Biotechniques. 2004 Jun;36(6):982-5.

5: Hou J.
Design of endonuclease restriction sites into primers for PCR cloning.
Bioinformatics. 2002 Dec;18(12):1690-1.

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Last update 07-Jun-2007, Rating n/a of 0 votes.