Bacterial cell culture

Bacterial cell culture general procedures:

Follow microbiology bacterial handling practice for all procedures.

- Streak agar plate from bacterial stock and grow overnight at 37c bacterial incubator.

- Transfer bacterial colony using sterile toothpick or small pipette tip into 5ml of appropriate culture medium (with antibotics).

- Vortex and mix to disturbe bacterial colony.

- Continue to incubate at 37c on a shaker for overnight or until desired OD value.

- Collect bacterial by centrifugation.

References:

Bacterial cell culture protocol

Agar plate preparation

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Last update 11-Aug-2007, Rating n/a of 0 votes.