Technique / Molecular Biology / RNAi siRNA gene silencing
siRNA experiments
siRNA experiments
siRNA experiment guide:
1. Always design 2-4 siRNA sequences for your experiment. An useful online siRNA design tool can be found at Ambion siRNA design tool
2. Chose siRNA with GC content around 40-55%.
3. Purify siRNA when synthesized in vitro.
4. Avoid degradation of siRNA by contamination of RNase.
5. Avoid use of antibiotics in cell culture during siRNA transfection.
6. Optimize siRNA transfection procedure by chosing right combination of siRNA transfection reagents, cell passages and culture conditions.
See How to optimize siRNA transfection experiment
Top Ten Ways to Optimize siRNA Delivery in Cultured Cells
7. Setup appropriate siRNA transfection positive controls, negative control and labeled siRNA for transfection optimization and troubleshooting.
Last update 12-Nov-2007, Rating of 0 votes.
RNA Interference of Gene Expression (RNAi) in Cultured Drosophila Cells
siRNA design tools from Whitehead Institute
siRNA sequence list (human, rat, mouse)
RNA Silencing Methods and Protocols
RNAi technique animation tutorial from Nature
siRNA transfection
Knock-down of endogenous proteins by RNA interference in HeLa cells
