Technique / Molecular Biology / Site-directed mutagenesis
Internal deletions by PCR
Internal deletions by PCR
I wonder if anybody could tip me on a good and fast way of making internal deletions in a plasmid by PCR. I have previously done it by introducing new restriction sites by site directed mutagenesis (and then digesting and re-ligating) which takes quite a long time... there ... ...
Last update 21-Nov-2000, Rating of 0 votes.
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