Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification
Protease inhibitors
Protease inhibitors
Summary: Protease inhibitors used in protein extraction step.
Content: Protease inhibitors essential for prevent protein extracts from proteolysis, dephosphorylation, and other protein degradation/modification related activities.
Commonly used protease inhibitors include:
- EDTA (chelated calcium and magnesium ions)
- Sodium orthovanadate (tyrosine protein phosphatases inhibitor)
- Sodium fluoride (serine/threonine protein phosphatases inhibitor)
- Aprotinin
- Leupeptin (lysosomal protease inhibitor)
- Pepstatin (aspartic proteases inhibitor)
- PMSF (protease inhibitor)
Some vendors also provide cocktails or tablets of protease inhibitors. (Sigma, Roche etc)
Last update 03-Nov-2008, Rating of 0 votes.
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Polymorphisms of the Z protein protease inhibitor and risk of venous thromboembolism: a meta-analysis. Br J Haematol. 2008 Oct;143(2):284-7
Relationship between temporary inhibition and structure of disulfide-linkage analogs of marinostatin, a natural ester-linked protein protease inhibitor. J Pept Res. 2005 Aug;66(2):49-58
Solution structure of marinostatin, a natural ester-linked protein protease inhibitor. Biochemistry. 2005 Feb 22;44(7):2462-8
[X-ray analysis of subtilisin complexed with an engineered protein protease inhibitor SSI] Tanpakushitsu Kakusan Koso. 1992 Feb;37(3 Suppl):208-9
Modification of the carboxypeptidase A active site residue Glu-270 prevents interaction with a protein protease inhibitor from Ascaris. Biochim Biophys Acta. 1980 Apr 11;612(2):384-94
[Stoichiometry of the reaction between the protein protease inhibitor from beans with trypsin and chymotrypsin] Dokl Akad Nauk SSSR. 1978 Aug 11;241(5):1214-6