Technique / Molecular Biology / Cloning library construction and screening


Cloning big fragments



Cloning big fragments

We have had a continuous problem in cloning large PCR fragments (5-8kb) into a smaller cloning vector (3-5kb). We have used various restriction enzyme site ends. Does anyone have any helpful hints/tricks for cloning 5-8kb PCR or non-PCR DNA fragments into conventional cloning sites in 3-5kb vectors? ... ...

Last update 05-Jan-2002, Rating of 0 votes.

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By prashanth chandraman on 05-Jan-2002
hi,

i have a problem in insertion of large fragment 7-8kb isolated from BAC to a small vector 3kb. we are doing all kind of possible methods but nothing going on. Does anyone have any helpful hints/tricks for cloning 5-8kb in 3-5kb vectors?


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By prashanth chandraman on 05-Jan-2002
hi,

i have a problem in insertion of large fragment 7-8kb isolated from BAC to a small vector 3kb. we are doing all kind of possible methods but nothing going on. Does anyone have any helpful hints/tricks for cloning 5-8kb in 3-5kb vectors?


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