Technique / Molecular Biology / DNA analysis techniques / DNA southern blot
Southern analysis with genomic fragment
Southern analysis with genomic fragment
I have to use a large fragment of DNA from a gen clone as a probe for a
Southern blot. I know it contain some repititive elementss. Is there a
protocol for pre-annealing the labelled DNA fragemn to block the repeatst
prior to hybridisation? Many thanks in advance
John
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Assuming your genomic fragment is human, try checking this Cot-1
competetive hyb info from Gibco BRL:
http://www2.lifetech.com/catalog/techline/molecular_biology/Manuals_PPS/15279011.pdf
It should work with mouse Cot-1 and genomic DNA, too
--Todd
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Last update 16-Jun-2008, Rating of 1 votes.
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