Technique / Proteomics and protein biochemistry / Chromatography HPLC purification


HPLC Trouble Shooting



HPLC Trouble Shooting

HPLC Trouble Shooting guide from Thomson Instrument Company.

  • Abnormal Peak Shape
  • Fluctuation in Elution time
  • Fluctuation in Baseline
  • Noise in Baseline
  • Rise in Pressure
    ... ...

    URL: HPLC Trouble Shooting Guide
    Contents: original figures/illustrations

    Last update 25-Jun-2003, Rating of 6 votes.

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    By NASEEM AHMAD on 05-Mar-2009
    how to be minimize the column back pressure
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    By MUTHUSWAMY on 03-Feb-2007
    excellent basic point of learning is from here only.
    please gine instruction on also sample processing and methods available for processing
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    By venkata rao on 01-Jan-2007
    very use full to the hplc users
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    By pramod dixit on 30-May-2006
    i want about hplc,s column basic fundamental for troubleshoting of hplc
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    By Mukesh prajapati on 27-Mar-2006
    i'm newcomer on HPLC that'why want the basic facts about hplc troubleshooting.
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    By PRAKASH SINGH on 19-Jun-2005
    want to know abt trouble shooting
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    By Ms Smita Ainapure on 09-Feb-2004
    During system suitability , every third injection area is going out of Relative Standard Deviation limit.
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    By Katthaleeya on 25-Jun-2003
    sample : plasma

    sample preparation : protein precipitation

    by acetonitrile:2-propanol (7:3)

    method of analysis : HPLC

    column C18

    mobile phase : methanol

    problem : negative peak and rise in pressure
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