Technique / Genetics / Cytogenetics analysis

Cytogenetics Lab Protocols

Write your comment

By rita on 11-Aug-2008 hola... mi inquietud deriva en cual es la diferencia entre el colcemid y colchicina, yo tengo un protocolo standarizado pero , no salen los cromosomas lo sufiecientemente alargados, he probado con varios tiempos 50 y 90 minut y diferntes cantidades de colcemid, pues ese es el reactivo que utilizo, 25 ul , 30ul. quiero aprender a reconocerlos, y he leido que es indispensable sean alrgados. Gracias de antemano rita Rating: n/a Reply

	By Diego Caraballo on 21-Apr-2008 Is there a way of preserving bone marrow cells in a specific buffer for some days, before starting the cell culture? What would the procedure be like? thanks. Rating: n/a Reply

	By Şebnem Ergünsu on 10-Aug-2007 Respected Sir, I am a biolog.I am working in cytogenetik lab.I am in trouble elongating the chromosomes.I don't want to use ethidium bromide ,I prefer diluting colcemid but the band level is not enough.please advise me a protocol for HR Chromosomes.Thank you from now on Rating: n/a Reply

	By PRANAV PANKAJ on 24-May-2007 respected sir.... i am using RPMI1640 for BM sample.during harvesting what will be the best time(how much) for kcl treatment. thanking u sir hope u will reply soon.... Rating: Very Good Reply

By rajsharma on 13-Mar-2003 Respected Sir, I am a medical graduate and doing research in medical genetics. I was able to get chromosomes from the peripheral blood . I am using RPMI 1640 medium. I had tried the same medium for products of conception and I was able to get only a few cells in the slide but there were no chromosomes. The same was the case with amniotic fluid. I am adding colcichine for 2 hours to the tissue on the 9 th day. Please advise me regarding the protocol for tissue culture . Thanking you in anticipation Raj sharma Rating: Good Reply

	By Dr. Amol on 02-Mar-2002 This website gives us basis protocol for cytogenetic analysis Rating: Good Reply