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Best way of cell cycle separation



Best way of cell cycle separation

What's the best way to separate cells based on cell cycle phase?
I read about something called "counterflow centrifugal elutriation", but have
no idea what it is. Can you actually separate cells based on cell cycle phase
using FACS or does it just detect. Basically, I want to have different pools
of cell enriched for each phase of the cell cycle.

Original source


FACScan can only detect, if you want to sort cells you have to have a cell
sorter. Alternatively cells can be separated according to their denisity in
a special centrifuge (G1 cells contain less DNA and are therefore less
dense.... than G2/M cells)

Original source

Last update 12-Jun-2002, Rating of 0 votes.

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By mingxing wu on 15-Mar-2004
I also want to perform cell sorting to separate cells from whole-culture. Cell sorting function in FACS is difficult to perform. Would you tell me how to soring different phase cells using cytometer? Thanks every reply me!
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By sami on 25-Sep-2002
I want some books for the piochimstry ,I hope from you send me :

1-the price .

2-the book name.

3-the transfer price.

thank you so mutch.


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By Nina on 12-Jun-2002
it's cool!
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