Technique / Cell Biology / Cell cycle analysis


Cell-cycle independent internal control genes



Cell-cycle independent internal control genes

I'm investigating cell cycle progression by measuring changes in the
amount of cyclin mRNA by RT-PCR. For this I need an internal control gene
which is cell-cycle independent, since this is not so for commonly-used
internal references such GAPDH or b-actin. Are there any opinions about
suitable internal standard genes which are cell cycle independent?
Martin

Last update 18-Aug-2005, Rating of 1 votes.

Post your message in Cell cycle analysis forum

Write your comment

By John Wilkinson on 18-Aug-2005
you might look into 18S rna - this is very abundant and often also used for normalization. I don't know if it fluctuates during the cell cycle
Rating:

Reply


Please Login or Register to Post