Technique / Molecular Biology / Enzymatic treatment of DNA/RNA


Double restriction digestion of DNA



Double restriction digestion of DNA

Double restriction digestion of DNA involves simultaneously DNA double digestion using two restriction enzymes. It is important to chose right enzymes with compatible buffers so that both enzymes have high activity. Most vendors provide restriction digestion buffer chart that allow users to check compatibility of different restriction digestion buffer system. NEB provides an online tool NEB double digestion enzyme finder to help users find compatible enzymes for double digestion. If there are difficulties to find compatible enzymes for double or even triple digestion, isoschizomers can be used and NEB has a extended list of Isoschizomers

Last update 18-Mar-2004, Rating of 0 votes.

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By Nisha on 18-Mar-2004
Hi, I was wondering how could we perform a double digest with enzymes that are at an optimum at different temperatures? For example the Sma1 with BamH1 and EcoRV with BstE11. Thanks!
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