Troubleshooting and Optimization of Retroviral Transduction Parameters
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Troubleshooting and Optimization of Retroviral Transduction Parameters (archive)
One of the most frequent laments of using recombinant retroviruses is that the titer does not appear to be sufficient to infect the cells of interest at a high enough frequency to suit your purposes.
This is a troubleshooting guide using Phoenix lines. (Nolan lab, Stanford University).
Last update 24-Jul-2001, Rating Good of 0 votes.
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Dear sir
i am working as grad. astt. in ULM school of Pharmacy in the Pharmacoogy deptt. for a doctoral program. i am involved wih transfection procedures in TSA cell line using the CalPo4 system. i amnot able to get good transfection coz the scintillation count is not significant. can u guide me towards conducting stable transfection. i don't get solid lumps of DNA ppt. upon adding cal soln into the HEBS soln instead i get a very fine ppt. highly disperesed and the sytem appears light milky. Kindly guide me... Soliciting cooperation
with regards......Srinivasan
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