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Cloning long DNA fragments inserts



Cloning long DNA fragments inserts

I had attempted to clone a 5kb insert (from a genomic library) into
the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with
nosuccess. I am doing this only to sequence my inserts. I do a blue
white screening, none of my white colonies contain the insert. One
of my colleagues suggested using the pUC 19 vector.

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Last update 14-Jan-2002, Rating Good of 0 votes.


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