Are my restriction enzymes cutting?

I have performed a digest on a vector (pCMV) to use in a ligation. How can I tell if the digest was successful? The difference in size of the undigested and digested vector is only 30 base pairs. I have not run on a gel because the undigested is 4.3 kb and I don't think that such a small difference in size will be noticable. Also, is there a way to tell if one enzyme worked- if I perform the two digests separately? As of now I have been doing the digests simultaneously with a buffer that is compatible for both enzymes.

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Last update 05-Oct-2005, Rating n/a of 1 votes.